1H, 13C, and 15N resonance assignment of the C terminal region of the disordered postsynaptic scaffold protein GKAP

Guanylate kinase-associated protein, GKAP, is a largely disordered scaffold protein with multiple interaction partners, playing a role in the organization of the postsynaptic protein network. Its C-terminus contains a binding motif for the PDZ domain of Shank proteins, another class of postsynaptic...

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Bibliográfiai részletek
Szerzők: Nagy-Kanta Eszter
Sánta Anna
Kálmán Zsófia Etelka
Li Jessica Amy
Permi Perttu
Gáspári Zoltán
Péterfia Bálint
Dokumentumtípus: Cikk
Megjelent: 2025
Sorozat:BIOMOLECULAR NMR ASSIGNMENTS 20 No. 1
Tárgyszavak:
Szerkezetbiológia (NMR)
Biokémia
doi:10.1007/s12104-025-10253-2

mtmt:36440658
Online Access:https://publikacio.ppke.hu/2858

MARC

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024 7 |a 10.1007/s12104-025-10253-2  |2 doi 
024 7 |a 36440658  |2 mtmt 
040 |a PPKE Publikáció Repozitórium  |b hun 
041 |a Angol 
100 2 |a Nagy-Kanta Eszter 
245 1 0 |a 1H, 13C, and 15N resonance assignment of the C terminal region of the disordered postsynaptic scaffold protein GKAP  |h [elektronikus dokumentum] /  |c  Nagy-Kanta Eszter 
260 |c 2025 
490 0 |a BIOMOLECULAR NMR ASSIGNMENTS  |v 20 No. 1 
520 3 |a Guanylate kinase-associated protein, GKAP, is a largely disordered scaffold protein with multiple interaction partners, playing a role in the organization of the postsynaptic protein network. Its C-terminus contains a binding motif for the PDZ domain of Shank proteins, another class of postsynaptic scaffolds. Based on predictions, this motif is preceded by a ~ 40-residue disordered segment with no known additional binding sites or established functional role. Here we report the expression, purification, and 1 H, 13 C and 15 N resonance assignment of the GKAP Ct43 construct containing the C-terminal 43 residues. This region is functionally intact, having a binding affinity to Shank1 PDZ in the micromolar range. Chemical shifts indicate that this region is indeed disordered but displays helical propensity in two regions. The short, slightly helical segment immediately before a PDZ-binding motif is somewhat comparable to the structural organization observed at the C-terminus of the E6 and RSK1 proteins, but its significance in GKAP is yet to be explored. 
650 4 |a Szerkezetbiológia (NMR) 
650 4 |a Biokémia 
700 0 1 |a Sánta Anna  |e aut 
700 0 1 |a Kálmán Zsófia Etelka  |e aut 
700 0 1 |a Li Jessica Amy  |e aut 
700 0 1 |a Permi Perttu  |e aut 
700 0 1 |a Gáspári Zoltán  |e aut 
700 0 1 |a Péterfia Bálint  |e aut 
856 4 0 |u https://publikacio.ppke.hu/id/eprint/2858/1/s12104-025-10253-2.pdf  |z Dokumentum-elérés  

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